Journal: bioRxiv

Article Title: Building the Marine Probiotic Platform: Developing a High-Throughput Process to Discover Coral Probiotics for Stony Coral Tissue Loss Disease

doi: 10.64898/2026.01.11.698879

Figure Lengend Snippet: Comparison of target strain growth in monoculture and co-culture conditions. (A, B) Relative fluorescence units (RFU) over time for target strains OfT6-21 with pBU164 (A) and McT4-56 with pBU164 (B) cultured in GASW-Tris broth in a 96-well microtiter plate. Each target strain was grown in monoculture (purple line with circles), in co-culture with its respective wildtype (WT) strain (blue line with diamonds), and in co-culture with the putative coral probiotic, Pseudoalteromonas sp. strain McH1-7 (green line with triangles) ( n = 10 per treatment). (B) Area under the curve (AUC) calculations for target strain OfT6-21 with pBU164 show significant differences in growth between the monoculture (purple circles), co-culture with its WT strain (blue diamonds), and co-culture with strain McH1-7 (green triangles) (one-way ANOVA; F(2, 27) = 37.90, p < 0.0001 , n = 10 per group). (D) AUC calculations for target strain McT4-56 with pBU164 show significant differences in growth across monoculture (purple circles), co-culture with its WT strain (blue diamonds), and co-culture with strain McH1-7 (green triangles) (one-way ANOVA; F(2, 27) = 316.2, p < 0.0001 , n = 10 per group). Asterisks indicate statistical significance (****= p ≤ 0.0001 ). Error bars represent the standard error of the mean (SEM), and bar height in the bar graphs represent the mean.

Article Snippet: To screen for antibacterial activity against the target strains, each tester culture (coral mucus isolate) was co-cultured with each target strain separately in a sterile black flat bottom 96-well plate (Cell culture microplate, PS, F-bottom, 655086, Greiner Bio-One).

Techniques: Comparison, Co-Culture Assay, Fluorescence, Cell Culture